GeneMapper ID-X Software v (AppliedBiosystems,FosterCity,California) mixtureanalysistool assists the forensicanalyst with the analysis and interpretation of mixed DNA samples. The mixture deconvolution module of the GeneMapper ID-X Software (GMID-X) evaluates two-person mixtures. GMID-X has the ability to produce best-fit major and minor. Overall, the GeneMapper® ID-X v software met the design requirements for analyzing mixture samples with 1, 2, and 3 or more contributors. Data from known mixtures demonstrated that the software effectively functions as an automated tool to deconvolute samples into the most likely major and minor contributors with or without known matching. Stutter filters set in GeneMapper® ID-X using the values below may be used to filter out potential stutter peaks. In a mixed source sample, stutter peaks may need to be considered. Refer to GeneMapper® Analysis Methods in this manual for the application of stutter filters in sample analysis. Stutter threshold values (% of alleles) are as follows.
GENEMAPPER ID – TROUBLESHOOTING GUIDE DATE EFFECTIVE APPROVED BY NUCLEAR DNA TECHNICAL LEADER PAGE 7 OF 16 Controlled versions of Department of Forensic Biology Manuals only exist in the Forensic Biology Qualtrax software. All printed versions are non-controlled copies. © NYC OFFICE OF CHIEF MEDICAL EXAMINER k. The GeneMapper® ID-X Software Version / Reference Guide: † Provides reference information on sizing and genotyping. † Describes the GeneMapper® ID-X Software quality value system and peak quality values (PQVs). Audience This guide is intended for GeneMapper ID-X Software users who review and evaluate sizing and genotyping data. 6 GeneMapper® ID-X Software Version Reference Guide Baseline Window Size The Baseline Window size parameter controls baselining for a group of peaks. The software determines a reference baseline value for each data point. In general, the software sets the reference baseline to be the.
GMID-X not only allows for the streamlining of data analysis, it also qualifies as an expert system by assisting in the manual review of single source samples. 28 thg 4, 5. Data analysis including color separation is performed semi-automatically through the use of. GeneMapper® ID-X software. For the. AmpFlSTR® Yfiler® PCR Amplification Kit User Guide. About This Guide. Data Collection and GeneMapper® ID or ID-X Software.
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